Publications

# indicates co-corresponding author
* indicates equal contribution (co-first author)

Publications from work done before joining Emory:

2025

Bou-Nader C., Juma K.M., Bothra A., Brasington A.J., Ghirlando R., Suzuki M., Garboczi D.N., Leppla S.H., Zhang J.
Structural basis of double-stranded RNA recognition by the J2 monoclonal antibody.
Nat Commun. 2025 Dec 13. doi: 10.1038/s41467-025-67414-z. Epub ahead of print. PMID: 41390480.
Gupta R., Gaikwad S., Qui H., Bou-Nader C., Zhang J., Hinnebusch A.G.
Purification and Analysis of eIF2α Phosphorylation by Stress-Activated Protein Kinase Gcn2 from S. cerevisiae.
Methods Mol Biol., 195-220.
Bou-Nader C., Link K.A., Suddala K.C., Knutson J.R., Zhang J.
Structures of complete HIV-1 TAR RNA portray a dynamic platform poised for protein binding and structural remodeling.
Nat Commun. Mar 6;16(1):2252.

Bou-Nader, C.*, Gaikwad, S.*, Bahmanjah, S.*, Zhang, F., Hinnebusch, A.G. and Zhang, J.
Gcn2 structurally mimics and functionally repurposes the HisRS enzyme for the integrated stress response.
Proc Natl Acad Sci U S A, 121.
Skeparnias, I., Bou-Nader, C., Anastasakis, D.G., Fan, L., Wang, Y.X., Hafner, M. and Zhang, J.
Structural basis of MALAT1 RNA maturation and mascRNA biogenesis.
Nat Struct Mol Biol.

Bou-Nader, C., Pecqueur, L., de Crecy-Lagard, V. and Hamdane, D.
Integrative Approach to Probe Alternative Redox Mechanisms in RNA Modifications.
Acc Chem Res., Online ahead of print.
Wei, E., Bou-Nader, C., Perry, M.L., Fattah, R., Zhang, J., Leppla, S.H. and Bothra, A. S9.6
Antibody-Enzyme Conjugates for the Detection of DNA-RNA Hybrids.
Bioconjug Chem, 34, 834-844.

Valencia-Gallardo, C., Aguilar-Salvador, D.I., Khakzad, H., Cocom-Chan, B., Bou-Nader, C., Velours, C., Zarrouk, Y., Le Clainche, C., Malosse, C., Lima, D.B. et al.
Shigella IpaA mediates actin bundling through diffusible vinculin oligomers with activation imprint.
Cell Rep, 42, 112405.

Bou-Nader, C.# and Zhang, J.#
Rational engineering enables co-crystallization and structural determination of the HIV-1 matrix-tRNA complex.
STAR Protoc, 3, 101056.

Bou-Nader, C., Bothra, A., Garboczi, D.N., Leppla, S.H. and Zhang, J.
Structural basis of R-loop recognition by the S9.6 monoclonal antibody.
Nat Commun, 13, 1641.

Bou-Nader, C.*, Stull, F.W.*, Pecqueur, L.*, Simon, P., Guerineau, V., Royant, A., Fontecave, M., Lombard, M., Palfey, B.A. and Hamdane, D.
An enzymatic activation of formaldehyde for nucleotide methylation.Nat Commun, 12, 4542.

Bou-Nader, C.*, Muecksch, F.*, Brown, J.B., Gordon, J.M., York, A., Peng, C., Ghirlando, R., Summers, M.F., Bieniasz, P.D.# and Zhang, J.#
HIV-1 matrix-tRNA complex structure reveals basis for host control of Gag localization.
Cell Host Microbe, 29, 1421-1436 e1427.

Featured on the cover of the journal

Bou-Nader, C.# and Zhang, J.#
Structural Insights into RNA Dimerization: Motifs, Interfaces and Functions.
Molecules, 25.

Valencia-Gallardo, C., Bou-Nader, C., Aguilar-Salvador, D.I., Carayol, N., Quenech'Du, N., Pecqueur, L., Park, H., Fontecave, M., Izard, T. and Tran Van Nhieu, G.
Shigella IpaA Binding to Talin Stimulates Filopodial Capture and Cell Adhesion.
Cell Rep, 26, 921-932 e926.

Hood, I.V., Gordon, J.M., Bou-Nader, C., Henderson, F.E., Bahmanjah, S. and Zhang, J.
Crystal structure of an adenovirus virus-associated RNA.
Nat Commun, 10, 2871.

Dozova, N., Lacombat, F., Bou-Nader, C., Hamdane, D. and Plaza, P.
Ultrafast photoinduced flavin dynamics in the unusual active site of the tRNA methyltransferase TrmFO.
Phys Chem Chem Phys, 21, 8743-8756.

Bou-Nader, C., Pecqueur, L., Barraud, P., Fontecave, M., Tisne, C., Sacquin-Mora, S. and Hamdane, D.
Conformational Stability Adaptation of a Double-Stranded RNA-Binding Domain to Transfer RNA Ligand.
Biochemistry, 58, 2463-2473.

Bou-Nader, C., Gordon, J.M., Henderson, F.E. and Zhang, J.
The search for a PKR code-differential regulation of protein kinase R activity by diverse RNA and protein regulators.
RNA, 25, 539-556.

Bou-Nader, C., Barraud, P., Pecqueur, L., Perez, J., Velours, C., Shepard, W., Fontecave, M., Tisne, C. and Hamdane, D.
Molecular basis for transfer RNA recognition by the double-stranded RNA-binding domain of human dihydrouridine synthase 2.
Nucleic Acids Res, 47, 3117-3126.

Hardouin, P., Velours, C., Bou-Nader, C., Assrir, N., Laalami, S., Putzer, H., Durand, D. and Golinelli-Pimpaneau, B.
Dissociation of the Dimer of the Intrinsically Disordered Domain of RNase Y upon Antibody Binding.
Biophys J, 115, 2102-2113.

Bou-Nader, C., Montemont, H., Guerineau, V., Jean-Jean, O., Bregeon, D. and Hamdane, D.
Unveiling structural and functional divergences of bacterial tRNA dihydrouridine synthases: perspectives on the evolution scenario.
Nucleic Acids Res, 46, 1386-1394.

Bou-Nader, C., Bregeon, D., Pecqueur, L., Fontecave, M. and Hamdane, D.
Electrostatic Potential in the tRNA Binding Evolution of Dihydrouridine Synthases.Biochemistry, 57, 5407-5414.

Bou-Nader, C., Pecqueur, L., Cornu, D., Lombard, M., Dezi, M., Nicaise, M., Velours, C., Fontecave, M. and Hamdane, D.
Power of protein/tRNA functional assembly against aberrant aggregation.
Phys Chem Chem Phys, 19, 28014-28027.

Selected among the 2017 PCCP HOT Articles
Bou-Nader, C., Cornu, D., Guerineau, V., Fogeron, T., Fontecave, M. and Hamdane, D.
Enzyme Activation with a Synthetic Catalytic Co-enzyme Intermediate: Nucleotide Methylation by Flavoenzymes.
Angew Chem Int Ed Engl, 56, 12523-12527.

Hamdane, D., Bou-Nader, C., Cornu, D., Hui-Bon-Hoa, G. and Fontecave, M.
Flavin-Protein Complexes: Aromatic Stacking Assisted by a Hydrogen Bond.
Biochemistry, 54, 4354-4364.
Bou-Nader, C.*, Pecqueur, L.*, Bregeon, D., Kamah, A., Guerineau, V., Golinelli-Pimpaneau, B., Guimaraes, B.G., Fontecave, M. and Hamdane, D.
An extended dsRBD is required for post-transcriptional modification in human tRNAs.
Nucleic Acids Res, 43, 9446-9456.